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. 2025 Jun 18;10(25):26913–26922. doi: 10.1021/acsomega.5c01905

2. Insights into the Characterization of Immobilized Lipases from the Literature.

adsorption on various support immobilization
immobilized lipase support enzymatic activity references
Streptomyces sp. A3301 lipase sponge 57.31 U·g–1
scrub pad 20 U·g–1
S. aureus ALA1 CaCO3 86%
celite 545 16%
silica gel 23%
B. stearothermophilus caCO3 89.33%
celite 545 38.33%
Optimal Conditions for Immobilization
B. stearothermophilus lipase 4500 U·g–1
S. aureus ALA1 lipase 3500 U·g–1
Thermal and pH Stability
  temperature (°C) pH  
immobilized Streptomyces sp. A3301 lipase on sponge 30 and 60 °C 7.0
immobilized B.stearothermophilus lipase on CaCO3 65% 40 °C for 1 h
immobilized S. aureus ALA1 lipase 62% activity at 78 °C 58% activity at a pH range of 6.0 to 11.0
immobilized lipases CALA and CALB from Candida antarctica 40 and 50 °C 6.0–8.0
immobilized Acinetobacter hemolyticus lipase on eggshell membrane 50% activity for 150 min at 50 °C, 120 min at 60 °C, and 80 min at 70 °C 7.0