3. Substrate Specificity of Free and Immobilized LipS.g .
| substrate | free LipS.g | immobilized LipS.g |
|---|---|---|
| TC4 | 92 ± 2 | 96 ± 2* |
| TC8 | 91.66 ± 3.21 | 92.33 ± 2.08 |
| TC18 | 100 ± 0 | 100 ± 0 |
| coconut oil | 88.33 ± 3.05 | 89.33 ± 3.05 |
| sesame oil | 87.33 ± 3.05 | 85.33 ± 2.51 |
| soybean oil | 84.66 ± 3.5 | 87.66 ± 2.51 |
| sunflower oil | 77.33 ± 2.08 | 79.33 ± 4.71 |
a
The lipase activity was expressed as a percentage, where the maximal activity was considered 100%. The activity at each time point was calculated using the following formula: activity (%) = (activity at time point/initial activity) × 100. Comparisons were performed between free and immobilized LipS.g with each substrate. Significance levels are indicated as p < 0.05 (*).