Figure 2.

Laminar localization of the PTH2 receptor in the dorsal horn of mouse spinal cord. PTH2 receptor immunoreactivity was detected with a green labeled secondary antibody and CGRP (a, b), isolectin B4 (c, d), protein kinase C gamma (e), and substance P (f), with red labeled secondary antibodies. High-power confocal microscopy indicates that there is virtually no colocalization between PTH2 receptor and CGRP (b) immunoreactivity or isolectin B4 labeling (d). [Bars = 100 μm (a, c, e, f), 20 μm (b, d).]