Table 1.
Recommended imaging parameters for the seven-color program
| Scan unit manufacturer and model | Leica STELLARIS 8; Leica SP8 |
Details |
|---|---|---|
| Acquisition Model | stage | Using the stage model |
| Sequential Scan | Between stacks, Sequential setting provided in Figure 2 | |
| xy | Format | 512∗512 for 20X; 1024∗1024 for 40X |
| Speed | 400 Hz | |
| Scan directionality | bidirectional | |
| Zoom factor | 1 | |
| Line average | 2 for 20X; 3 for 40X | |
| Pinhole | 1 A.U. | |
| z-stack | z-stack size | 1.5 μm |
| Stage | Filed size | According to experimental requirements |
| Tile Scan Stitching Overlap | x: 10%, y: 10% | |
| Accessories | laser | 405 nm; WLL2 (440 nm–790 nm) |
| Objective | 20X (NA 0.75, Air); 40X (NA 1.3, oil) | |
| Detector | Power HyD S |