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. 2025 Apr 16;6(2):103776. doi: 10.1016/j.xpro.2025.103776

Table 2.

Evaluating of staining effectiveness and adjustment recommendations based on signal-to-noise ratio

Target protein Background Potential solutions
Strong signal Weak signal Good performance
Strong signal Strong signal
  • 1.

    Reduce the staining time.

  • 2.

    Adjust the antibody incubation temperature from room temperature to 4°C.
Weak signal Strong signal
  • 1.

    Optimize the antibody dilution ratio.

  • 2.

    Perform staining following antigen retrieval
Weak signal Weak signal This antibody may not be suitable for immunofluorescence