REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
eFluor 450 anti-mouse CD326 (EpCAM) monoclonal antibody (clone G8.8)	eBioscience	Cat# 48-5791-82, RRID:AB_10717090
BV480 rat anti-mouse CD4 (clone RM4-5)	BD Biosciences	Cat# 565634, RRID:AB_2739312
Alexa Fluor 488 rat anti-mouse CD4 (clone RM4-5)	BD Biosciences	Cat# 557667, RRID:AB_396779
Alexa Fluor 647 rat anti-mouse CD4 (clone RM4-5)	BD Biosciences	Cat# 557681, RRID:AB_396791
Alexa Fluor 594 anti-mouse CD326 (Ep-CAM) antibody (clone G8.8)	BioLegend	Cat# 118222, RRID:AB_2563322
Alexa Fluor 488 anti-mouse CD3 antibody (clone 17A2)	BioLegend	Cat# 100210, RRID:AB_389301
Alexa Fluor 647 anti-mouse CD31 antibody (clone MEC13.3)	BioLegend	Cat# 102516, RRID:AB_2161029
Alexa Fluor 488 anti-mouse F4/80 monoclonal antibody (clone BM8)	eBioscience	Cat# 53-4801-82, RRID:AB_469915
BV480 rat anti-mouse CD45R/B220 (clone RA3-6B2)	BD Biosciences	Cat# 565631, RRID:AB_2739311
CD45R (B220) monoclonal antibody (RA3-6B2), eFluor 450	eBioscience	Cat# 48-0452-82, RRID: AB_1548761
Alexa Fluor 647 anti-mouse CD8a antibody (clone 53–6.7)	BioLegend	Cat# 100724, RRID: AB_389326
CD4 monoclonal antibody (RM4-5), Alexa Fluor 700	eBioscience	Cat# 56-0042-82, RRID:AB_494000
Alexa Fluor 488 anti-mouse LYVE1 monoclonal antibody (clone ALY7)	eBioscience	Cat# 53-0443-82, RRID:AB_1633415
LYVE1 monoclonal antibody (ALY7), eFluor 450	eBioscience	Cat# 48-0443-80, RRID: AB_2784722
Alexa Fluor 488 anti-GFP antibody	BioLegend	Cat# 338008, RRID: AB_2563287
eFluor 570 anti-mouse Foxp3 monoclonal antibody (clone FJK-16s)	eBioscience	Cat# 41-5773-82, RRID:AB_11219073
Alexa Fluor 647 mouse anti-Ki-67 (clone B56)	BD Biosciences	Cat# 561126, RRID:AB_10611874
Alexa Fluor 700 mouse anti-Ki-67 (clone B56)	BD Biosciences	Cat# 561277, RRID:AB_10611571
BV480 rat anti-mouse CD8a (clone ALY7)	BD Biosciences	Cat# 566096, RRID:AB_2739500
Goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 647	Invitrogen	Cat# A-21244, RRID:AB_2535812
Chemicals, peptides, and recombinant proteins
Fixation and permeabilization solution	BD Biosciences	Cat# 554722
Normal goat serum	Jackson ImmunoResearch Laboratories, Inc.	Cat# 005-000-121, RRID: AB_2336990
Normal mouse serum	Jackson ImmunoResearch Laboratories, Inc.	Cat# 015-000-120, RRID: AB_2337194
Normal rabbit serum	Jackson ImmunoResearch Laboratories, Inc.	Cat# 011-000-120, RRID: AB_2337123
Normal rat serum	Jackson ImmunoResearch Laboratories, Inc.	Cat# 012-000-120, RRID: AB_2337141
PBS	HyClone	Cat# SH30256.01
Triton X-100	Sangon Biotech	Cat#A600198-0500
Tissue-Tek O.C.T. compound	Sakura Finetek	Cat# 4583
Sucrose	Sigma-Aldrich	Cat# v900116
Fluoromount-G	SouthernBiotech	Cat# 0100-01
DAPI	Sigma-Aldrich	Cat# D9542
Bovine serum albumin	Sigma-Aldrich	Cat# B2064-100G
Experimental models: Organisms/strains
Mouse: ApcMin/+	The Jackson Laboratory	JAX: 002020
Mouse: C57BL/6	The Jackson Laboratory	JAX: 000664
Mouse: Foxp3DTR	The Jackson Laboratory	JAX:016958
Software and algorithms
Prism 8	GraphPad	https://www.graphpad.com/
Imaris 10.0	Bitplane	https://imaris.oxinst.com/
R 4.3.2	N/A	https://www.r-project.org/
Python 2.7	N/A	https://www.python.org/
Leica LAS X Suite software	Leica	N/A
Other
Charged slides	Premiere	Cat# 9308W
Standard NorGlas microscope cover glass	MeVid	Cat# CS01-2450
Reagent reservoirs, 7 mL, 8 channels	Sangon Biotech	Cat#F619713-0001
Hydrophobic Barrier PAP Pen	Vector	Cat# H-4000
Leica CM1950 cryostat	Leica	CM1950
Leica TCS SP8	Leica	N/A
Leica STELLARIS 8 STED	Leica	N/A