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. 2025 Apr 16;6(2):103776. doi: 10.1016/j.xpro.2025.103776

Fixation buffer

Reagent Final concentration Amount
Fixation and Permeabilization Solution N/A 1 mL
PBS N/A 2 mL
Total N/A 3 mL

Total 3 mL fixation buffer per intestine. Prepare before use.