Table 2.
Plant and seed components of transgenic wheat plants expressing or not expressing the altered maize AGP large subunit (SH2R6HS)
| Generation† | Group‡ | Heads per plant
|
Seeds per plant
|
Seed weight per plant, g
|
Average individual seed weight, mg
|
Harvest index
|
Total plant biomass
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| + | − | + | − | + | − | + | − | + | − | + | − | |||
| T0 | Mean§ | 6.0 | 5.3 | 198.5 | 138.5 | 5.85 | 4.67 | 28.7 | 32.2 | 0.457 | 0.577 | 11.7 | 7.7 | |
| (2.6) | (2.5) | (109.1) | (74.0) | (3.22) | (2.56) | (2.2) | (3.3) | (0.092) | (0.022) | (6.5) | (4.4) | |||
| +/−¶ | 1.13 | 1.43 | 1.25 | 0.89 | 0.79 | 1.52 | ||||||||
| T2 | 152-7 | 7.5 | 44.1 | 1.34 | 29.8 | 0.180 | 6.4 | |||||||
| 155-4 | 5.2 | 56.6 | 1.70 | 29.8 | 0.255 | 5.7 | ||||||||
| 161-8 | 6.0 | 38.8 | 1.30 | 33.2 | 0.222 | 5.1 | ||||||||
| 161-12 | 5.9 | 71.7 | 2.50 | 34.8 | 0.310 | 7.2 | ||||||||
| 152-10 | 4.0 | 27.1 | 0.76 | 27.7 | 0.160 | 3.8 | ||||||||
| 161-1 | 5.8 | 52.6 | 1.80 | 34.1 | 0.256 | 6.2 | ||||||||
| 152-2 | 5.7 | 30.3 | 1.06 | 33.7 | 0.159 | 5.0 | ||||||||
| 152-9 | 5.1 | 39.8 | 1.32 | 32.2 | 0.255 | 4.4 | ||||||||
| Mean§ | 6.1 | 5.3 | 55.6 | 39.1 | 1.78 | 1.30 | 31.6 | 32.3 | 0.246 | 0.210 | 6.2 | 5.1 | ||
| (1.7) | (1.9) | (26.5) | (28.7) | (0.91) | (1.03) | (3.0) | (3.8) | (0.079) | (0.107) | (1.7) | (2.1) | |||
| +/−¶ | 1.15** | 1.42** | 1.37** | 0.98 | 1.17* | 1.22** | ||||||||
| F2‖ | Mean§ | 6.5 | 4.7 | 186.5 | 137.2 | 6.48 | 4.70 | 35.4 | 33.8 | 0.594 | 0.553 | 10.3 | 7.9 | |
| (1.7) | (1.1) | (42.2) | (42.2) | (1.33) | (1.65) | (6.4) | (4.9) | (0.031) | (0.091) | (1.8) | (1.8) | |||
| +/−¶ | 1.38** | 1.36** | 1.38** | 1.05 | 1.07 | 1.31** | ||||||||
For the T0 generation, n = 4 for both the positive and the negative group. For the T2 generation, n = 60 and 57 for the positive and negative groups, respectively. The 60 positives consist of 17 152-7, 20 155-4, 6 161-8, and 17 161-12. The 57 negatives consist of 12 152-10, 20 161-1, 15 152-2, and 10 152-9. Individual transgenic lines are from the T2 generation. For the F2 generation, n = 14 and 9 for plants homozygous positive and negative for SH2R6HS, respectively.
Numbers in + columns for T0 and T2 generations are pooled data for lines expressing SH2R6HS (152-7, 155-4, 161-8, and 161-12). Numbers in − columns for T0 and T2 generations are pooled data for lines lacking expression (152-2, 152-9, 152-10, and 161-1). Numbers in + and − columns for individual transgenic lines from the T2 generation are for lines expressing or lacking expression of SH2R6HS, respectively.
Group means are accompanied by standard deviations in parentheses.
For the F2 generation, numbers in + and − columns are progeny from a 161-12 × HL cross that are homozygous positive and negative for SH2R6HS, respectively.
+/− ratios are determined by dividing the positive group mean by the negative group mean.
,
, and
denote P < 0.05, 0.01, and 0.001, respectively.