Appendix 1—table 2. Lipids and metabolites upregulated in low or high tachyplesin-nitrobenzoxadiazole (NBD) accumulators compared to untreated bacteria.

Lipid or metabolite name, composition, adduct, retention time (RT), theoretical and experimentally measured mass, formula, variable importance in projection (VIP), and concentration in parts per million (∆ppm) measured via quadrupole time-of-flight liquid chromatography/mass spectrometry.

Name	Composition	Adduct	RT	Theoretical mass	Experimental mass	Formula	VIP Score	∆ppm
Lipids and metabolites upregulated in low tachyplesin-NBD accumulators
LPE 20:4	-	[M+H]	1.49	502.2928	502.2925	C25 H44 NO7 P	1.46	–0.59
SM 34:1	-	[M+H]	3.40	703.5749	703.5751	C39 H79 N2 O6 P	1.30	0.28
PE 32:1	-	[M+H]	3.20	689.4996	689.4997	C37 H72 NO8 P	1.48	0.14
PE 36:5	-	[M+H]	3.25	738.5069	738.5067	C41 H72 NO8 P	1.60	–0.27
PE 38:5	-	[M+H]	3.40	766.5382	766.5380	C43 H76 NO8 P	1.56	–0.26
SM 34:1	18:1; 16:0	[M-H]	3.8	701.5603	701.5603	C39 H79 N2 O6 P	1.97	0.28
LPC 18:1	-	[M-H]	1.09	520.3408	520.3406	C26 H52 NO7 P	1.23	–0.38
Lipids and metabolites upregulated in high tachyplesin-NBD accumulators
Cer 32:332:3	14:2; 18:1	[M+H]	3.32	506.4568	506.456745	C32 H59 NO3	1.37	–0.78
PC 36:3	18:0; 18:3	[M+H]	3.74	788.5851	788.5849	C44 H82 NO8 P	1.42	–0.25
LPC 22:1	-	[M+H]	1.19	578.418	578.4160	C30 H60 NO7 P	1.46	–3.45
SM 40:2	16:1; 24:1	[M+H]	3.48	785.6531	785.6560	C45 H89 N2 O6 P	1.52	3.69
PE 30:0	16:0; 14:0	[M+H]	3.08	664.4912	664.4910	C35 H70 NO8 P	1.64	–0.30
PE 31:0	16:0; 15:0	[M+H]	3.19	678.5069	678.5067	C36 H72 NO8 P	1.71	–0.29
PE 32:2	16:1; 16:1	[M+H]	3.21	688.4912	688.4910	C37 H70 NO8 P	1.63	–0.29
LPC 18:1	-	[M-H]	1.09	520.3408	520.3403	C26 H52 NO7 P	1.91	–0.96
SM 34:1	18:1; 16:0	[M-H]	3.8	701.5603	701.5601	C39 H79 N2 O6 P	1.97	–0.28
PC 35:0	-	[M-H]	2.49	774.6018	774.6016	C43 H86 NO8 P	1.26	–0.25
PG 38:7	-	[M-H]	1.63	791.4868	791.4861	C44 H73 O10 P	1.63	–0.88
PS 37:7	20:5; 17:2	[M-H]	5.69	790.4664	790.4667	C43 H70 NO10 P	1.19	0.37