KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| N49P9 | This study | N/A |
| N49P9.1 | This study | N/A |
| N49P9.3 | This study | N/A |
| N49P9.6 | This study | N/A |
| N49P9.3-FR | This study | N/A |
| N49P9.6-FR-LS | This study | N/A |
| eN49P9 | This study | N/A |
| PGT121 | This study | RRID: AB_2491041 |
| Synagis | Medimmune LLC | RRID: AB_2459638 |
| Bacterial and virus strains | ||
| 119 HIV-1 pseudovirus “global panel” | (Seaman et al.)81; this study | N/A |
| Biological samples | ||
| Human CD34 Cells | Lonza | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| HIV BaL gp120 | (Fouts et al.)82; this study | N/A |
| HIV BG505 SOSIP.664 | (Sanders et al.)83; this study | N/A |
| HIV A/E gp12093TH057coree | (Acharya et al.2014)84; this study | N/A |
| HIV CH505TFchim.6R.SOSIP.664v4.1 | Dr. Barton Haynes, Duke University | N/A |
| Critical commercial assays | ||
| Protein A agarose | GE Healthcare | Cat#20333 |
| TaqMan Fast Virus 1-Step master mix | Thermo Fisher | Cat#4444432 |
| Deposited data | ||
| Crystal Structure N49P9.1 | This study | PDB: 6OZ3 |
| Crystal Structure N49P9.3 | This study | PDB: 7SX6 |
| Crystal Structure N49P9.3-FR | This study | PDB: 7SX7 |
| Cryo-EM Structure PGT121 and N49P9.6-FR3 | This study | PDB: 7UOJ |
| Cryo-EM map of PGT121 and N49P9.6-FR3 | This study | EMDB: EMD-26648 |
| Sequence of N49P9 | 26, This study | GenBank:MG819641 (heavy chain) MG819646 (light chain) |
| Sequence of N49P9.3 | This study | GenBank:PV344714.1 (light chain) PV344713.1 (heavy chain) |
| Sequence of PGT121 | 80, This study | GenBank:JN201911 (light chain) JN201894 (heavy chain) |
| Experimental models: Cell lines | ||
| TZM-bl cells | NIH AIDS Reagent Program | Cat#8129 |
| Freestyle 293-F cells | Thermo Scientific | Cat #R79007 |
| CEM.NKRCCR5 cells | Dr. John Kappes | N/A |
| HD-BIOP3 GS Null CHO K1 | Revvity | N/A |
| Experimental models: Organisms/strains | ||
| scid FcRn−/− hFcRn | Jackson Laboratories | Strain #018441 |
| NSG-SGM3 | Jackson Laboratories | Strain #013062 |
| NSG-IL15 | Jackson Laboratories | Strain #030890 |
| Oligonucleotides | ||
| 6F 5’- CAT GTT TTC AGC ATT ATC AGA AGG A -3’ | Palmer et al.85 | N/A |
| 84R 5’-TGC TTG ATG TCC CCC CAC T-3’ | Palmer et al.85 | N/A |
| FAM-P 5’- /56-FAM/CCA CCC CAC /ZEN/AAG ATT TAA ACA CCA TGC TAA /31ABkFQ/-3’ | Palmer et al.85 | N/A |
| Software and algorithms | ||
| Prism (v5.0) | GraphPad | http://www.graphpad.com |
| CryoSPARC | Structural Biotechnology Inc. | https://cryosparc.com |
| REFMAC | Murshudov et al.86 | http://www.ccp4.ac.uk/html/refmac5/description |
| PHENIX | Adams et al.87 | https://www.phenix-online.orq |
| ChimeraX | Meng et al.88 | https://www.rbvi.ucsf.edu/chimerax |
| COOT | Guan et al.89, University of Cambridge | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| MolProbity | Davis et al.90, Duke University | molprobity.biochem.duke.edu |
| PPP4 Suite | Collaborative Computational Project91, Science and Technology Facilities Council | www.ccp4.ac.uk/ |
| PyMol Molecular Graphic suite | DeLano Scientific | http://pymol.org |
| PISA Webserver | Protein Data Bank in Europe77 | www.ebi.ac.uk/pdbe/pisa |
| SAS software version 9.4 | SAS Institute Inc. | https://www.sas.com |