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. 2002 Feb 12;99(4):1905–1909. doi: 10.1073/pnas.042700399

Figure 1.

Figure 1

Preparation of site-specific DNA–protein crosslinks. (A) Sequence of the uracil-containing 60-mer oligodeoxynucleotide. (B) Urea-PAGE showing DNA substrate preparation. Lane 1, uracil-containing 60-mer; lane 2, uracil-containing 60-mer, digested with uracil DNA glycosylase and tested with T4-pdg (control of AP-site formation); reduced AP site-containing DNA before (lane 3) and after (lanes 4–6) purification; DPC-containing DNA before (lane 7) and after (lanes 8–10) purification. After purification, DNAs were subjected to the restriction endonuclease digestion with SnaBI (5, 9) or HaeIII (6, 10). (C) SDS/PAGE showing DPC-containing DNA substrates before (lane 1) and after (lane 2) HaeIII digestion.