Table 1.

Kinetic parameters for CjPglB and DmPglB with GalNAc₅(Glc)GlcNAc LLOs

ssOST	Acceptor sequon	kcat (h−1)	KM (μM)
CjPglB	DQNAT	0.42 ± 0.08	10.7 ± 0.98
DmPglB	DQNAT	0.33 ± 0.05	4.30 ± 0.95
CjPglB	QYNST	n.a.	n.a.
DmPglB	QYNST	0.24 ± 0.02	5.16 ± 1.13

^aK_M and k_cat values calculated from in vitro glycosylation data shown in Supplementary Fig. 9. Reactions were performed using extracted GalNAc₅(Glc)GlcNAc LLOs and fluorescent TAMRA-GSDQNATF-NH₂ or TAMRA-GQYNSTAF-NH₂ as substrates. Data are the average of technical replicates (n = 3) ± SD. In the case of CjPglB with QYNST sequon, no activity (n.a.) was detected.