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. 2002 Feb 5;99(4):1943–1948. doi: 10.1073/pnas.042688399

Figure 1.

Figure 1

The morphology and the binding of FITC-labeled annexin V to various resealed membrane preparations. (A) Dark-field light microscopy of control ghosts (a), MgATP-ghosts (b), and MgAMPPNP-ghosts (c). Control and MgAMPPNP-ghosts are echinocytic whereas MgATP ghosts are discocytic. (B) Representative cytofluorograph histograms of various resealed membrane preparations after binding of FITC-labeled annexin V. Histograms in gray represent background fluorescence whereas histograms in black represent specific binding. Control ghosts (a) and MgAMPPNP-ghosts (c) exhibit significant binding of annexin V whereas there is no binding of annexin V to MgATP-ghosts (b).