Figure 3.

Examples of immunofluorescence of centrosomes stained for centrin (green) in normal breast tissue (A) and centrosome amplification in breast tumors (B–E) and for microtubule nucleation (F–H) (green, anti-tubulin). (A) Region of a normal breast duct (lumen, center right) showing nuclei (red) located in the basal region of epithelial cells and pairs of centrioles (green, anti-centrin) located apically. (B–E) Examples of breast tumors showing the range of centrosome amplification in tumor tissue: (B) Diploid tumor. (C) Monosomy 17 tumor. (D) Stable aneuploid tumor. (E) Unstable aneuploid tumor. (F–H) Three examples of microtubule nucleation in touch preparations of breast tumor cells: (F) Diploid tumor. (G) Monosomy 17 tumor. (H) Stable aneuploid tumor.