Table 1.
Statistical analysis of noise in measurements of fluorescent beads
| Beads* | LSCM | FACS† | WFM/Raw | WFM/Decon | FACS‡ |
|---|---|---|---|---|---|
| .3 | .44 | .09 | .35 | .24 | .20 |
| 1 | .15 | .07 | .09 | .10 | .10 |
| 3 | .18 | .05 | .08 | .08 | .08 |
| 10 | .37 | .06 | .09 | .08 | .07 |
| 30 | .24 | .06 | .07 | .08 | .06 |
| 100 | .13 | .05 | .06 | .07 | .06 |
Values are coefficients of variation (standard deviation/arithmetic mean) for each of the bead populations measured in Fig. 2. Between 30 and 45 beads were used for each bead population assayed by microscopy. 1200–1500 beads from each population were used for each FACS assay (see Methods).
*
Nominal relative bead fluorophore density as specified by manufacturer.
†
Coefficients of variation for beads used for LSCM measured by FACS.
‡
Coefficients of variation for beads used for WFM measured by FACS.