Table 1.
Summary of the eye phenotypes of embryos that are injected with RNA for eyeless, twin of eyeless, and an activated form of Notch receptor at the 2-cell stage
| Normal | Duplication | Eye expansion | Other | Dead | Total | |
|---|---|---|---|---|---|---|
| ey | ||||||
| 100 pg | 91% (29) | 0% (0) | 0% (0) | 9% (3) | 0% (0) | 32 |
| 250 pg | 44% (14) | 44% (14) | 6% (2) | 6% (2) | 0% (0) | 32 |
| 500 pg | 23% (22) | 23% (22) | 39% (37) | 12% (11) | 2% (2) | 94 |
| 1 ng | 0% (0) | 2% (1) | 91% (58) | 6% (4) | 2% (1) | 64 |
| toy | ||||||
| 100 pg | 100% (32) | 0% (0) | 0% (0) | 0% (0) | 0% (0) | 32 |
| 500 pg | 87% (82) | 2% (2) | 0% (0) | 4% (4) | 6% (6) | 94 |
| 1 ng | 31% (10) | 50% (16) | 0% (0) | 13% (4) | 6% (2) | 32 |
| 2 ng | 6% (2) | 81% (26) | 3% (1) | 0% (0) | 9% (3) | 32 |
| Notch E | ||||||
| 1 ng | 30% (11) | 22% (8) | 0% (0) | 43% (16) | 5% (2) | 37 |
| 5 ng | 38% (26) | 26% (18) | 0% (0) | 29% (20) | 6% (4) | 68 |
Embryos were injected with eyeless (ey), twin of eyeless (toy), and an activated form of Xenopus Notch receptor (Notch) RNA into one blastomere at the 2-cell stage and allowed to develop to stage 41 to 42. Typical examples of the duplication of eyes and eye expansion are presented in Figs. 1F and 2A, respectively. The other phenotypes are mainly caused by head defects.