Table 2.
Summary of the phenotypes of embryos that are injected with RNA for eyeless, twin of eyeless, and an activated form of Notch receptor at the 16-cell stage
| Normal | Ectopic RPE | Eye defect | Other | Dead | Total | |
|---|---|---|---|---|---|---|
| ey | ||||||
| 100 pg | 86% (49) | 2% (1) | 2% (1) | 0% (0) | 11% (6) | 57 |
| 125 pg | 68% (40) | 15% (9) | 14% (8) | 2% (1) | 2% (1) | 59 |
| 250 pg | 25% (14) | 25% (14) | 38% (21) | 5% (3) | 13% (7) | 56 |
| toy | ||||||
| 100 pg | 91% (48) | 0% (0) | 0% (0) | 2% (1) | 8% (4) | 53 |
| 500 pg | 80% (90) | 3% (3) | 9% (10) | 1% (1) | 9% (10) | 113 |
| notch_E | ||||||
| 500 pg | 83% (45) | 0% (0) | 11% (6) | 4% (2) | 2% (1) | 54 |
| 2 ng | 86% (96) | 0% (0) | 8% (9) | 3% (3) | 5% (5) | 111 |
Embryos were injected with eyeless (ey), twin of eyeless (toy), and an activated form of Xenopus Notch receptor (Notch) RNA into one animal pole blastomere at the 16-cell stage and allowed to develop to stage 48. Typical examples of the ectopic RPE and the proximal eye defect are presented in Fig. 3 A and D, respectively. The other phenotypes are mainly caused by head defects.