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Change in [Na+]i in response to SMIF. Sperm were loaded with cell permeant NaGi and were suspended in cuvettes containing 1/2 FSW; baseline fluorescence readings were taken before SMIF was added. SMIF produced a decrease in fluorescence intensity (n = 9), signifying a depolarization event. This decrease (12%) was significantly greater (P < 0.05) than that observed when 1/2 FSW alone was added to sperm suspensions (1% decrease).
