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Efflux of Na+ from sperm in response to Ca2+. Sperm were suspended in 1/2 NaCaF containing the cell impermeant Na+ probe NaGo. After establishing a baseline fluorescence, Ca2+ (5 mM final) was added to the medium. An increase in fluorescence occurred, signifying an efflux of Na+ (Δ = 0.59 mM) from sperm (n = 3). This efflux was blocked by bepridil (100% with 10 μM) and partially inhibited by flunarazine (51% with 20 μM).
