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Effect of SMIF on membrane potential. Sperm were suspended in 1/2 FSW or preincubated with 20 μM bepridil or 50 μM nifedipine for 5 min, followed by the addition of DiSC3(5) and CCCP (not shown). Upon addition of SMIF (arrow), a depolarization was observed (increase in fluorescence intensity) which was inhibited slightly by nifedipine (6%) and more so by bepridil (67%). No change in membrane potential was observed in sperm to which 1/2 FSW was added.
