Figure 2.

Effect of CHAMP on fetal gene expression in neonatal cardiomyocytes. Primary neonatal rat cardiomyocytes were infected with adβ-gal or adCHAMP for 2 h. After infection, the culture medium was replaced with serum-free medium. Cells were incubated for a further 24 h at which time PE (20 μg/ml) was added as indicated. Total RNA was isolated 48 h later, and RNA dot blots were probed with oligonucleotide probes for the indicated genes. Expression of each transcript was quantified by phosphoimaging and is expressed as the level of expression relative to untreated cultures. Expression levels were normalized to the level of expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which was used as an internal control for RNA loading (not shown). The adβ-gal had no effect on the up-regulation of hypertrophic marker genes by PE. The results are the average ± SD of three independent experiments.