Table 1.
Trapping of foreign DNA during transformation with hybrid molecules
| Donor DNA type of event | R304 (chromosomal) point mutation transfer | pR313 ccc plasmid integration* | Linear pR313 InsDel |
|---|---|---|---|
| SmR ml−1 | 0.99 × 106 | — | — |
| MTXR ml−1 | — | 1.45 × 106 | 2.02 × 104 |
| EryR ml−1 | — | 1.31 × 106 | 8.6 × 103 |
| EryR/MTXR | — | 0.90 | 0.47 |
*
The most frequent event occurring with covalently closed circles (ccc) donors is integration of the entire plasmid (34). InsDel represent 5% of total events with ccc donors (unpublished observations).