Figure 5.

Styx interacts with Crhsp-24 in vivo. (A) Expression of selected proteins in whole adult mouse testis. Testes proteins were isolated and resolved by SDS/PAGE. Selected proteins were detected by commercial antibodies. Relative positions of molecular mass standards are in kDa. (B Upper) Crhsp-24 is selectively retained in anti-Styx immune complexes from wild-type testes. Increasing amounts (1, none; 2, 15 μg; 3, 40 μg) of anti-Styx antibodies were used to immunoprecipitate proteins for each lysis condition (Nonidet P-40, high salt, and RIPA). After removing the immune complexes, the unbound lysate material was combined (Ext. Lysate), and all proteins were resolved by SDS/PAGE and Western blots probed with antibodies as described above. (Lower) Styx is retained in Crhsp-24 immune complexes from wild-type testes. Immune precipitation was performed as described above by using affinity-purified Crhsp-24 antisera.