Table 1.
Breeding scheme for producing F2 segregants
Generation | Function | B6 × bm1 | B6 × bm3 | bm1 × bm3 |
---|---|---|---|---|
P | Mix inbred genomes | B6/B6 × bm1/bm1 | B6/B6 × bm3/bm3 | bm1/bm1 × bm3/bm3 |
F1 | Create F2 segregants | B6/bm1 × B6/bm1 | B6/bm3 × B6/bm3 | bm1/bm3 × bm1/bm3 |
F2 | Collect urine from homozygous F2 males | B6/B6, B6/bm1, and bm1/bm1 | B6/B6, B6/bm3, and bm3/bm3 | bm1/bm1, bm1/bm3, and bm3/bm3 |