Table 1. Effect of calcium concentration during root induction on the growth of acclimated plantlets after transplantation.

Calcium concentration (mM)	Survival rate (%)	Shoot length (cm)	Number of fresh leaves
3	38.9	13.8±2.4	3.0±1.1
6	76.9	15.1±2.6	2.8±0.9
p-value	0.067a	0.33b	0.69b

Shoot segments prepared as mentioned in Figure 1, transferred to 1/2MS media containing 2% (w/v) sucrose, 3 mM or 6 mM Ca²⁺, 0.5 mg l⁻¹ indole-3-butyric acid, 0.125% (w/v) gellan gum [(2)/2MS2CIB0.5 or (2)/2MS4CIB0.5, respectively], and incubated at 15°C under 14 h light period (Pale Pink LED, 110–160 µmol m⁻² s⁻¹). Rooted plantlets (PLKD2) were cold-treated at 4°C for 72 days, then transplanted to a closed greenhouse [20°C, 60% relative humidity, 16 h light period (sunlight and supplemental LED light)]. Survival rate, shoot length, and number of fresh leaves were observed 76 days after transplantation. The values represent the mean±SD of at least seven replicates. ^a Fisher’s exact test, ^b Student’s t-test.