Figure 3.

RNA blot analysis of HrpL-dependent expression of representative virulence-implicated genes. Each well was loaded with 25 μg of total RNA isolated from CUCPB5114 cultures carrying either vector control pCPP5031 or P_nptII-hrpL plasmid pCPP5032 (lanes 2 and 3, respectively). PCR-amplified internal fragments were used as probes; lane 1 in each case contains PCR product of the corresponding probe. AvrPpiB11_Pto and AvrPpiB12_Pto are 100% identical, therefore their signals cannot be distinguished.