KEY RESOURCES TABLE
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Anti-guinea pig complement C3 goat IgG fraction, FITC | MP Biomedicals | Cat # 0855385, RRID:AB_2334913 |
Anti-human IgG1 PE [HP6001] | Southern Biotech | Cat #9054–09, RRID:AB_2796628 |
Anti-human IgG3 PE [HP6050] | Southern Biotech | Cat #9210–09, RRID:AB_2796701 |
Biological samples | ||
LowTox Guinea Pig Complement | CedarLane Labs | Cat # CL4051 |
Chemicals, peptides, and recombinant proteins | ||
Human soluble FcγR2A | Duke University | Custom Order |
Human soluble FcyR3A | Duke University | Custom Order |
LC-LC-Sulfo-NHS Biotin | ThermoFisher | Cat # A35358 |
Streptavidin-R-PE | Prozyme | Cat # PJ31S |
Recombinant Epstein-Barr virus 1–90 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus 377–459 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus 460–641 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus 29–84 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus 365–420 B95-8 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus 393–448 B95-8 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus 393–448 AG876 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus EBNA-1 421–476 B95-8 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus EBNA-1 589–641 B95-8 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus EBNA-1 589–641 AG876 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus EBNA-1 1–56 GD1 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus EBNA-1 1–56 B95-8 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus EBNA-1 421–476 AG876 | SinoBiological | Custom Synthesis |
Recombinant Epstein-Barr virus EBNA-1 449–504n B95-8 | SinoBiological | Custom Synthesis |
Influenza AH1N1(A/Ohio/UR06-0091/2007) | SinoBiological | Custom Synthesis |
Ebolavirus glycoprotein (GP) (Subtype Bundibugyo, strain Uganda 2007) GP1/Glycoprotein histag(40368-V08B) | SinoBiological | Custom Synthesis |
CRYAB (aa 1–27): MDIAIHHPWIRRPFFPFHSPSRLFDQFGGGEQKLISEEDLGGGMDIAIHHPWIRRPFFPFHSPSRLFDQFGGGHHHHHH | Alan Scientific | Custom Synthesis |
GlialCAM (aa 370–389): ATGRTHSSPPRAPSSPGRSRGGGEQKLISEEDLGGGATGRTHSSPPRAPSSPGRSRGGGEQKLISEEDLHHHHHH | Alan Scientific | Custom Synthesis |
ANO2 (aa 140–149): PGDIELGPLDGGGEQKLISEEDLGGGPGDIELGPLDGGGEQKLISEEDLGG HHHHHH | Alan Scientific | Custom Synthesis |
Myc control (spacer): EQKLISEEDL GGG EQKLISEEDL GGG EQKLISEEDL GGG EQKLISEEDLGGG HHHHHH | Alan scientific | Custom Synthesis |
Critical commercial assays | ||
NHS-Sulfo-LC-LC Kit | ThermoFisher | 21435 |
Zeba-Spin Desalting and Chromatography Columns | ThermoFisher | 89882 |
EDC | ThermoFisher | 22980 |
Deposited data | ||
Luminex and Flow Cytometry MFI Data | GitHub | HSPHSystemsSerology/ProjID_KG20241203) |
Experimental models: Cell lines | ||
THP-1 monocytes | ATCC | ATCC TIB-202/RRID: CVCL_0006 |
Software and algorithms | ||
iQue Forecyt 9.1 | Sartorius | 60028 |
R Studio V 4.0 | R Project for Statistical Computing | RRID:SCR_000432 |
Other | ||
384-well HydroSpeed Plate Washer | Tecan | 30190112 |
IntelliFlex Luminex Dual-Report | Luminex | APX2021 |
iQue Screener Plus | Intellicyt/Sartorius | 11811 |
MagPlex Microspheres | Diasorin | MC12001-01 (Cataloged by region) |
Scarlet Fluorescent Neutravidin Microspheres | ThermoFisher | Custom Synthesis |
Green Fluorescent Neutravidin Microspheres | ThermoFisher | Custom Synthesis |
Red Fluorescent Neutravidin Microspheres | ThermoFisher | Custom Synthesis |