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MMTV-induced B cell activation is independent of viral gene expression. (A) MMTV (FM) was treated with 5 mM AT-2, DMSO vehicle, or untreated and injected into C3H/HeN mice (n = 3). Draining and nondraining lymph nodes were isolated at 18 h and 4 days postinjection, stained for CD69 and B220 expression, and analyzed by FACS. (B) Percentage of CD69/B220+ lymphocytes in AZT-treated mice injected with MMTV (LA). D, draining lymph node; ND, nondraining lymph node.
