3. Reactive Carbonyl Species and Modification Sites Identified in Human Serum Albumin as a Result of Reactive Electrophiles.
| Modification Site | Distinct Modification Count | Modification Mass Shifts | Subdomain | Lipid-Binding Site |
|---|---|---|---|---|
| C58 | 3 | +355.131, +369.147, +399.121 | IA | No |
| K214 | 3 | +312.089, +369.147, +383.138, +381.147 | IIA | Site 9 |
| K219 | 4 | +312.089, +383.138, +395.138, +409.142, +457.163, | IIA | Site 8 |
| K223 | 11 | +312.089, +355.131, +367.131, +369.147, +371.126, +383.138, +385.142, +395.138, +399.121, +409.142, +419.126, +475.174 | IIA | Site 7/Site 8 |
| K456 | 2 | +312.089, +395.138 | IIIA | Site 9 |
| K460 | 1 | +395.138 | IIIA | Site 9 |
| K543 | 3 | +312.089, +383.138, +395.138 | IIIB | No |
| K549 | 6 | +355.131, +383.138, +385.142, +395.138, +419.126, +475.174 | IIIB | Site 5 |
| K565 | 4 | +312.089, + 369.147, + 383.138, + 395.139 | IIIB | No |
| K588 | 1 | +355.131 | IIIB | No |
a
Modification site relative to the canonical sequence of HSA (Uniprot ID: P02768).
b
Mass shifts corresponding to unknown ARP-reactive carbonyl species.
c
Based on proximity, no defined electron density to map amino acid orientation.