Table 7.
| Reagent | Volume (μL) |
|---|---|
| DAPI | 0.75 |
| Concanavalin A AF750 | 0.75 |
| anti-MAP-2 Antibody AF594 (BioLegend, 801802) | 1.5 |
| anti-(Thr308)-pAKT Antibody | 1.5 |
| 20% Triton-X 100 | 9.375 |
| Cell Painting Buffer: 1X HBSS + 1% BSA + 0.01%NaN3 | 750 |
| total | 763.875 |
| Reagent | Volume (μL) |
|---|---|
| DAPI | 0.75 |
| Concanavalin A AF750 | 0.75 |
| anti-MAP-2 Antibody AF594 (BioLegend, 801802) | 1.5 |
| anti-(Thr308)-pAKT Antibody | 1.5 |
| 20% Triton-X 100 | 9.375 |
| Cell Painting Buffer: 1X HBSS + 1% BSA + 0.01%NaN3 | 750 |
| total | 763.875 |