Table 2.

Statistical table of assembly results.

Sample ID	Contig numbers	Total length (bp)	Largest length (bp)	N50 (bp)	GC (%)	Mapped (%)
DM1	542,147	663,647,397	19,067	1,802	36.81	99.16
DM2	576,213	656,313,157	17,411	1,612	36.80	99.07
DM3	586,992	655,410,243	21,887	1,559	36.86	99.13
DM4	600,988	648,912,080	16,911	1,476	36.79	98.74
DM5	560,055	652,035,236	21,724	1,666	36.79	98.98
DM6	541,951	749,569,994	31,721	2,342	37.43	99.44
SB1	572,327	659,045,269	21,904	1,660	36.37	99.17
SB2	585,496	656,769,512	16,863	1,596	36.30	99.10
SB3	578,067	646,948,786	62,921	1,585	36.40	98.61
SB4	570,251	668,883,798	24,264	1,724	36.37	99.06
TY1	346,293	567,719,182	30,601	2,884	38.14	98.85
TY2	299,638	564,673,231	46,385	3,716	38.07	99.43
TY3	299,168	569,806,024	54,071	3,901	38.03	99.45
TY4	310,963	565,928,600	34,725	3,499	38.05	99.15

DM, Diptychus maculatus; SB, Schizothorax biddulphi; TY, Triplophysa yarkandensis. Contig numbers: the number of contigs after assembly. Total length: the sum of the bases of all contigs. Largest length: the longest contig base number. N50 is defined as the length of the contig at which the cumulative length first reaches or exceeds half of the total length of all contigs. (Contigs are arranged in descending order based on their lengths, and the cumulative length is calculated progressively.) Mapped: the ratio of sequencing reads to assembled contigs.