Table 2. Golgi residence times of transmembrane secretory cargos and Golgi glycosyltransferases in native Golgi (without nocodazole).
Superscripts 1 and 2 indicate data were from previous publications, (Sun et al., 2020) and (Sun et al., 2021), respectively. The two RUSH reporters, SBP-GFP-CD59 and TNFα-SBP-GFP, employ signal sequence fused streptavidin-KDEL and Ii-streptavidin as the ER hook, respectively. n, the number of quantified cells; SEM, standard error of the mean.
| Transmembrane protein | Golgi residence time (t1/2) | n | SEM |
|---|---|---|---|
| TNFα-SBP-GFP1 | 6.0 min | 73 | 0.4 min |
| GFP-CD8a1 | 7.8 min | 26 | 0.7 min |
| TfR-GFP1 | 10 min | 12 | 1 min |
| GFP-Tac1 | 16 min | 45 | 2 min |
| SBP-GFP-CD59 | 16 min | 29 | 2 min |
| GFP-Tac (5 A)1 | 47 min | 57 | 3 min |
| GFP-CD8a-TC | 66 min | 19 | 7 min |
| GFP-Tac-TC1 | 3.4 h | 22 | 0.3 h |
| MGAT2-GFP2 | 4.9 h | 26 | 0.5 h |
| ST6GAL1-GFP1 | 5.3 h | 21 | 0.6 h |