Figure 1.

Expression of Gα_gust and G_t-2 in rat GI tissues and a gastric endocrine cell cDNA library. Consensus primers to amplify Gα_gust and Gα_t-2 were designed based on the published rat, mouse, and human sequences, as shown in Table 1. PCR amplification was performed on reverse-transcribed poly(A)⁺ RNA isolated from rat antrum (A), fundus (F), and duodenum (D), and on cDNA from a gastric endocrine cell library (GEC). The predicted sizes of the PCR products of Gα_t-2 (Top) and Gα_gust (Middle) are 340 bp and 332 bp, respectively. A cDNA fragment (764 bp) corresponding to β-actin was amplified as a control transcript from the respective cDNA sample (Bottom).