Two-component analysis of the νsymCH2 band in the infrared spectra of the thylakoid membranes RT35/100 (A), RT25/100 (B), and a cytoplasmic membrane RC35/100 (C) to illustrate the case of extremely high polar carotenoid concentration in a membrane. Thick solid lines indicate the measured spectra. The fitted component bands are indicated with thinner lines, except for the component assigned to the rigid fatty acid population, which is drawn with a thicker line. It should be noted that the relative intensity of this component band increases with the amount of polar carotenoids in the membranes. For details, see the text. Both the fitted component bands and the residual curves have been displaced for clarity.