TABLE 3.
IC50 values for the three drugs against 1.0 µM Mpro wild type and variantsa
| Nirmatrelvir | Ensitrelvir | Bofutrelvir | |
|---|---|---|---|
| Wild type (1.0 µM) | 390 nM (300 to 490) |
400 nM (320 to 500) |
420 nM (320 to 540) |
| T21I | 590 nM (570 to 620 nM) |
700 nM (590 to 830) |
780 nM (610 to 970) |
| L50F | 310 nM (270 to 350 nM) |
320 nM (280 to 360) |
710 nM (ND to 780) |
| S144A | 360 nM (320 to 410 nM) |
740 nM (620 to 870) |
460 nM (380 to 550) |
| E166A | 440 nM (410 to 470 nM) |
1.0 µM (900 nM to 1.1 µM) |
560 nM (440 to 700 nM) |
| E166V | 13 µM (10. to 15 µM) |
420 nM (340 to 500 nM) |
630 nM (590 to 660 nM) |
| E166I | 120 µM (98 to 140 µM) |
3.6 µM (3.1 to 4.1 µM) |
630 nM (570 to 690 nM) |
| E166L | 550 nM (520 to 580 nM) |
3.4 µM (3.0 to 4.0 µM) |
370 nM (350 to 400 nM) |
| QM (T21I/L50F/S144A/E166V) |
150 µM (140 to 160 µM) |
5.1 µM (4.0 to 6.5 µM) |
810 nM (740 to 900 nM) |
a
The IC50 values are based on data plotted in Fig. 2A through C, which summarize results from at least three replicates. The 95% confidence intervals estimated by GraphPad Prism are shown in parentheses. ND, not determined.