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. 2002 Feb 26;99(5):2613–2618. doi: 10.1073/pnas.052707099

Figure 1.

Figure 1

PPARδ represses transcription and interacts with corepressors. (A) PPARδ represses basal transcription. CV-1 cells were transfected with GAL4 DBD or GAl4-PPAR fusions. Normalized luciferase activity was 8.94 for GAL4 DBD, 12.58 for GAL4-PPARα, 9.45 for PPARγ, and 0.19 for PPARδ. Fold repression was determined relative to the GAL4 DBD activity. (B) PPARδ interacts with SMRT and NCoR. CV-1 cells were transfected with GAL4-SMRT RID (G4-SMRT) or NCoR RID (G4-NCoR) together with VP or VP-PPARδ. (C) PPARδ LBD interacts with SMRT. CV-1 cells were transfected with G4-SMRT and VP, VP-PPARδ, or VP-PPARδ LBD (VP-δLBD). (D) The ID1 of SMRT interacts with PPARδ. Transfection was performed with VP-PPARδ and GAL4 DBD or GAL4 fusion of SMRT ID1, ID2, and three ID1 mutants.