Figure 7.

PPARδ represses PPARγ signaling. (A) PPARδ represses PPARγ target gene expression. Total RNA was isolated from NIH 3T3-Babe, NIH-PPARγ, or NIH-PPARγ-PPARδ cells. The RNA blot was probed with PPARδ, PPARγ, aP2, CD36, or glyceraldehyde-3-phosphate dehydrogenase sequentially. (B) PPARδ inhibits PPARγ-mediated adipogenesis. NIH-PPARγ or NIH-PPARγ-PPARδ cells were cultured in standard (C) or differentiation media (DM). After 7 days of differentiation, cells were fixed and stained with oil red O.