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. 2025 Jun 26;12:1616977. doi: 10.3389/fmed.2025.1616977

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Copyright © 2025 Wang and Liu.

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(a) A translucent, elongated gelatinous object next to a ruler for scale. (b) A metal kidney dish with a ruler beside it. (c) A pink-stained fibrous pattern under a microscope. (d) A blue-stained fibrous pattern under a microscope. (e) Another pink-stained fibrous pattern under a microscope. (f) A black-and-white image of a dense fibrous network under a microscope. — Characteristics of DFSB. (a) Fish swim bladder (gross appearance). (b) After decellularization procedure, the DFSB appeared as a translucent film (gross appearance). (c) H&E staining of DFSB (x400). (d) Masson’s trichrome staining of DFSB (x 400). (e) Sirius Red staining of DFSB (x400) (f) Scanning electron microscope of DFSB (x1000).