Table 1.
Step | Total units, μmol min−1 | Total protein, mg | Specific activity, μmol min−1mg−1 | Yield, % |
---|---|---|---|---|
Cell-free extract | 679 | 842 | 0.8 | 100 |
40–65% (NH4)2SO4 | 622 | 504 | 1.2 | 92 |
DEAE-Sepharose | 384 | 36 | 10.6 | 57 |
Step | Total units, μmol min−1 | Total protein, mg | Specific activity, μmol min−1mg−1 | Yield, % |
---|---|---|---|---|
Cell-free extract | 679 | 842 | 0.8 | 100 |
40–65% (NH4)2SO4 | 622 | 504 | 1.2 | 92 |
DEAE-Sepharose | 384 | 36 | 10.6 | 57 |