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. 2002 Feb 26;99(5):2702–2707. doi: 10.1073/pnas.052527799

Table 1.

Purification of nitroalkane oxidase from E. coli

Step Total units, μmol min−1 Total protein, mg Specific activity, μmol min−1mg−1 Yield, %
Cell-free extract 679 842 0.8 100
40–65% (NH4)2SO4 622 504 1.2 92
DEAE-Sepharose 384 36 10.6 57