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Equilibrium unfolding of Hsp104549–908. (A) CD spectrum of a 40 μM solution of Hsp104549–908. Urea denaturation of Hsp104549–908 (B) and Hsp104549–908 Y819W (C) monitored by CD at 225 nm. (D) Urea denaturation of Hsp104549–908 Y819W monitored by fluorescence at 365 nm.
