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In vivo phenotypes of hsp104R826M. (A) Heterozygous mutant diploids grown on yeast extract/peptone/dextrose (YPD) at 30°C for 5 days. (B) Meiotic progeny from the HSP104/hsp104R826M diploid grown on YPD at 30°C for 5 days. (C) Recovery of wild-type and mutant strains from increasing exposure to 52°C. Five-fold serial dilutions of treated cells, allowed to recover at 30°C for 3 days, are shown.
