Figure 2.

Survivorship and MSRA expression driven by Ubi and GMR. (A and B) Fluorescent micrographs of the Ubi-GAL4/+; UAS-MSRA^C/+ and GMR-GAL4/+; UAS-MSRA^C/+ larvae. GFP fluorescence was detected diffusely in the whole body, especially in muscle fibers of Ubi-GAL4/+; UAS-MSRA^C/+ (A) and primarily confined to the eye area of the eye-antennal discs of GMR-GAL4/+; UAS-MSRA^C/+ (B). (Scale bar, 100 μm.) O, optic lobe; B, brain hemisphere; V, ventral ganglia; E, eye-antennal disk; M, muscle fiber. Ectopic expression of EGFP-MSRA was achieved by crossing MSRA^C with the homozygous Ubi-GAL4 or GMR-GAL4 activator line. (C) Survivorship curves of Ubi-GAL4/+; UAS-MSRA^C/+ (●) compared with the parental lines Ubi-GAL4 (□) and UAS-MSRA^C (○). The smooth lines represent best fits of the data using the Gompertz survivor function. The estimated lifespan values for the male and female animals in the Ubi-GAL4 group were 45 and 51 days, respectively. The values in the Ubi-GAL4/+; UAS-MSRA^C/+ were 64 and 70 days. The values of R₀ and α for MSRA^C, Ubi-GAL4/Ubi-GAL4 and Ubi-GAL4/+; UAS-MSRA^C/+ animals were 5.46 × 10⁻³/5.89 × 10⁻², 1.10 × 10⁻³/8.73 × 10⁻², and 2.08 × 10⁻⁵/1.29 × 10⁻² for males and 4.01 × 10⁻³/6.15 × 10⁻², 3.28 × 10⁻⁴/1.05 × 10⁻¹, and 1.57 × 10⁻⁴/8.37 × 10⁻³ for females. (D) Survivorship curves of GMR-GAL4/+; MSRA^C/+ (●) compared with the parental lines GMR-GAL4 (□) and UAS-MSRA^C (○). The smooth lines represent best fits of the data using the Gompertz survivor function. The estimated lifespan values for the male and female animals in the GMR-GAL4 group were 45 and 51 days, respectively. The values in the GMR-GAL4/+; MSRA^C/+ were 64 and 70 days. The values of R₀ and α for MSRA^C/MSRA^C, GMR-GAL4/GMR-GAL4, and GMR-GAL4/+; MSRA^C/+ animals were 5.46 × 10⁻³/5.89 × 10⁻², 3.78 × 10⁻⁴/1.23 × 10⁻², and 4.60 × 10⁻⁴/9.53 × 10⁻² for males and 4.01 × 10⁻³/6.15 × 10⁻², 1.65 × 10⁻³/6.77 × 10⁻², and 6.50 × 10⁻⁴/8.32 × 10⁻² for females. The control data in C and D are the same.