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. 2002 Mar 5;99(5):2760–2765. doi: 10.1073/pnas.052706199

Table 1.

Solubility in vivo

Protein Original sequence Lys in N-term. β-strand Lys in C-term. β-strand Double mutant
4-6 Inclusion body Inclusion body Inclusion body 50% soluble
50% incl. body
17-6 Inclusion body Soluble Inclusion body Soluble
45-8 Inclusion body Soluble Inclusion body Soluble