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Secondary structure determined by circular dichroism spectroscopy
| Protein | Original sequence | Lys. in N-term. β-strand | Lys in C-term. β-strand | Double mutant |
|---|---|---|---|---|
| 4-6 | β | β | β | β |
| 17-6 | β | β | β | Disordered |
| 45-8 | β | β | β | β |
A single minimum at 217 nm indicates β-sheet secondary structure. Sample spectra are shown in Fig. 3.
