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. 2005 Sep 22;115(10):2843–2854. doi: 10.1172/JCI25256

Figure 1.

Figure 1

Dietary PUFAs suppress the hepatic abundance of ChREBP mRNA and ChREBP total and nuclear protein content. (A) RTQ-PCR analysis of glycolytic and lipogenic genes from livers of 24 hour–fasted mice and mice refed a HCHO-triolein or HCHO-PUFA diet for 18 hours were performed. Results are the mean ± SEM; n = 6/group. *Significantly different from mice refed a HCHO diet for 18 hours (P < 0.005). (B) Insulin-stimulated liver lysates from 18 hour HCHO-, HCHO-triolein–, and HCHO-PUFA–fed mice blotted with anti–phospho-Akt (P-Akt) and anti–phospho-MAPK antibodies. Blots were then stripped and reprobed for total Akt and MAPK. n = 3/group. (C) Total, cytosolic, and nuclear ChREBP, precursor SREBP-1 (pSREBP-1), and mature SREBP-1 (mSREBP-1) protein, in cytosolic and nuclear extracts from livers of 24 hour–fasted and 18 hour–refed mice on a HCHO diet supplemented or not with PUFAs. β-Actin and Lamin A/C antibodies were used as loading controls. A representative Western blot is shown. n = 6/group.