Figure 2.

Impairment of Cat L or Cat S activity does not lead to accumulation of I-A^g7 molecule–associated Ii remnants. (A) Immunoblot for Ii on thymic extracts derived from Cat L–deficient or wild-type mice of H-2^b or H-2^g7 haplotype. (B) NOD- or C57BL/6-derived splenocytes were pulsed and chased in the presence or absence of LHVS. Samples were immunoprecipitated for class II MHC molecules or (C) for Ii with rabbit polyclonal antiserum. Formation of SDS-stable class II MHC complexes was assessed under mildly denaturing conditions (nonboiled) as indicated. The subunits of class II MHC molecules (α and β) and complexes with peptide (αβp) or Ii remnants (αβSLIP) are indicated. Ii isoforms (p31 and p41) and remnants (SLIP) are shown.