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. 2002 Feb 26;99(5):2866–2871. doi: 10.1073/pnas.052709799

Figure 2.

Figure 2

Biochemical properties of Nrdp1 and its domains. (A) Illustration of FLAG-tagged constructs used in these studies. (B) Stability and detergent extractability of Nrdp1. MCF7 cells were transfected with the FLAG-tagged constructs illustrated in A, and cells were treated without or with MG132 for 18 h as indicated. Cells were lysed in RIPA (R, 0.15 mM NaCl/0.05 mM Tris⋅HCl, pH 7.2/1% Triton X-100/1% sodium deoxycholate/0.1% SDS) or an Nonidet P-40-containing buffer (N). Proteins in lysates were immunoblotted with anti-FLAG epitope.