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PIASxβ catalyzes its own modification.35S-labeled in vitro translated PIASxβ or PIASxβC362S was incubated either in the absence (−) or presence (+) of the assay mix containing recombinant E1 (Aos1/Uba2), Ubc9, and SUMO-1. GST-PIASxβ (100 ng) was added where indicated.
