Table 1.
Oligonucleotide Sequence |
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Process and Exon | Forward | Reverse | AnnealingTemperature(°C) |
Genomic DNA amplification: | |||
1 | F-GGAGTTCGCGCGACGACCGC | R-CAAGAATCTATGCTAGGCAAC | 60 |
2 | F-GATGACAGTACCCGTCTCCAG | R-GCACTGACGTGGACTGAAAGTC | 60 |
3 | F-GAGTTTGGCACAGAGGAGGC | R-CTTTGGCTAGCATGGGTGATGC | 60 |
4 | F-CATGAGCCTGCGCTGTGCTC | R-GACAGTGGCTTACAGAAGCTG | 60 |
5 | F-GACAGTGGCTTACAGAAGCTG | R-CATCACAATAGAGTGGAAATC | 60 |
dHPLC screening: | |||
1 | F-GCGGACAGGCAGCAGTACTTG | R-CAAGAATCTATGCTAGGCAAC | 60 |
RT-PCR: | |||
1–5 | F-GAGTCCTGGAGCGCACAGAGAAGATG | R-CAGCTCATCTAGGTTGCTGTCGATC | 60 |
Quantitative real-time PCR: | |||
3–4 | F-GAAGCTATAAGTACAAGTAAAG | R-CATCAGTACTCATGGCAGAAC | 55 |