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. 2005 Jun 20;77(2):242–251. doi: 10.1086/432556

Table 1.

SNAP29 Oligonucleotide Sequences

Oligonucleotide Sequence
Process and Exon Forward Reverse AnnealingTemperature(°C)
Genomic DNA amplification:
 1 F-GGAGTTCGCGCGACGACCGC R-CAAGAATCTATGCTAGGCAAC 60
 2 F-GATGACAGTACCCGTCTCCAG R-GCACTGACGTGGACTGAAAGTC 60
 3 F-GAGTTTGGCACAGAGGAGGC R-CTTTGGCTAGCATGGGTGATGC 60
 4 F-CATGAGCCTGCGCTGTGCTC R-GACAGTGGCTTACAGAAGCTG 60
 5 F-GACAGTGGCTTACAGAAGCTG R-CATCACAATAGAGTGGAAATC 60
dHPLC screening:
 1 F-GCGGACAGGCAGCAGTACTTG R-CAAGAATCTATGCTAGGCAAC 60
RT-PCR:
 1–5 F-GAGTCCTGGAGCGCACAGAGAAGATG R-CAGCTCATCTAGGTTGCTGTCGATC 60
Quantitative real-time PCR:
 3–4 F-GAAGCTATAAGTACAAGTAAAG R-CATCAGTACTCATGGCAGAAC 55