Table 2.

SLC6A4 SNP Marker Information

							Primers		Probes
Marker Number	Marker Type	SLC6A4 Region	dbSNPa/Celera Number or Allele	Alleles	MAF	Intermarker Distance(bp)	Forward	Reverse	VIC	FAM	Product Size (bp)	Assay
1	SNP	Promoter 1A	rs1050565hCV7473213	T/C	.32	10,035	NA		NA	NA	NA	AoD
2	SNP	Promoter 1A	rs2020930 hCV11424041	G/A	.03	1,734	5′-GCTCAAGCAGGTGAACAAAGAAA-3′	5′-CTGGGCAGCTGGGAAGAG-3′	5′-VIC-AACTATTGCTATGCGGTGAT-MGB-NFQ-3′	5′-FAM-TTGCTGTGCGGTGAT-MGB-NFQ-3′	72	AbD
3	Ins/Del	Promoter 1A	5-HTTLPR	528(L)/484(S)	.44	2,549	5′-GGCGTTGCCGCTCTGAATGC-3′	5′-GAGGGACTGAGCTGGACAACCAC-3′			484/528	Gel
4	SNP	Intron 1A	rs2020933 hCV11424045	A/T	.07	295	5′-TGTATGTATTTTTACCATCAGTTTTGTCCAGAA-3′	5′-GAGAGTTAGCTAGCAGGCTCATAAAT-3′	5′-VIC-CATTGACCAGGTTCAC-MGB-NFQ-3′	5′-FAM-CATTGACCTGGTTCAC-MGB-NFQ-3′	81	AbD
5	SNP	Intron 1A	rs2020934 hCV7911197	C/T	.47	5	5′-TTTTCCTGCCACGCACTCT-3′	5′-GCACAAACCTCATAAGAACCTGCTT-3′	5′-VIC-ACCGTTCCAATATGG-MGB-NFQ-3′	5′-FAM-CCGTTCCAACATGG-MBG-NFQ-3′	80	AbD
6	SNP	Intron 1A	rs2020935 hCV11424046	T/A	.07	11,477	5′-TGGCAGTGACCGTTCCAA-3′	5′-TTGCTCAATTTGCACAAACCTCAT-3′	5′-VIC-CTGCTTCTCACTCATCCA-MGB-NFQ-3′	5′-FAM-TGCTTCTCACTCAACCA-MGB-NFQ-3′	68	AbD
7	SNP	Intron 1A	rs25528 hCV1841705	A/C	.16	80	5′-CCCAGTGGAGGCACAGG-3′	5′-GAGTGTGCAGGTTACTGATGCT-3′	5′-VIC-TGGTTGGTGTCGCCG-MGB-NFQ-3′	5′-FAM-TGGTTGGTTTCGCCG-MGB-NFQ-3′	62	AbD
8	SNP	Exon 1B	rs6354 hCV1841706	A/C	.17	931	5′-GGAGGCAAGGCGACCTT-3′	5′-CTGTGGCTAAGCCCCTTGTTATT-3′	5′-VIC-CTTGCCCTCTATTGCAG-MGB-NFQ-3′	5′-FAM-TTGCCCTCTCTTGCAG-MGB-NFQ-3′	58	AbD
9	SNP	Exon 2	Thr4Ala	A/G		157	5′-GTCATTTACTAACCAGCAGGATGGA-3′	5′-CGCTGATAGCTGCTTCTGAGA-3′	5′-VIC-ATTCAAGGGCGTCGTC-MGB-NFQ-3′	5′-FAM-CAAGGGCGCCGTC-MGB-NFQ-3′	62	AbD
10	SNP	Exon 2	Gly56Ala	G/C	.01	310	5′-GGGTACTCAGCAGTTCCAAGTC-3′	5′-GGGATAGAGTGCCGTGTGT-3′	5′-VIC-CTGGTGCGGGAGAT-MGB-NFQ-3′	5′-FAM-CTGGTGCGGCAGAT-MGB-NFQ-3′	56	AbD
11	VNTR	Intron 2	VNTR	9/10/12	.02/.39/.59	3,269	5′-TGGATTTCCTTCTCTCAGTGATTGG-3′	5′-TCATGTTCCTAGTCTTACGCCAGTG-3′			345/360/390	Gel
12	SNP	Exon 4	Lys201Asn	G/C		40	5′-GCTATACTACCTCATCTCCTCCTTCAC-3′	5′-TGGTGCAGTTGCCAGTGTT-3′	5′-VIC-CCAGGAGTTCTTGCAGC-MGB-NFQ-3′	5′-FAM-CAGGAGTTGTTGCAGC-MGB-NFQ-3′	83	AbD
13	SNP	Exon 4	Glu215Lys	G/A		1,802	5′-TCCTGGAACACTGGCAACTG-3′	5′-GAATGGAGGGTCCAGGTGATG-3′	5′-VIC-AATTACTTCTCCGAGGACAA-MGB-NFQ-3′	5′-FAM-AATTACTTCTCCAAGGACAA-MGB-NFQ-3′	65	AbD
14	SNP	Intron 5	rs140700 hCV7473202	G/A	.07	195	5′-ACTCCAAGGGTTGTGATCTTTCTG-3′	5′-GGGTGAATGGATGTCAGTGTCTTTT-3′	5′-VIC-ACCACCTCACCCTCCT-MGB-NFQ-3′	5′-FAM-CACCTCGCCCTCCT-MGB-NFQ-3′	89	AbD
15	SNP	Exon 6	Ser293Phe	C/T		505	5′-GTGACAGCCACCTTCCCTTATATC-3′	5′-GTGGCACCCCTCACCAG-3′	5′-VIC-CAGGACAGAAAGGAT-MGB-NFQ-3′	5′-FAM-CAGGACAAAAAGGAT-MGB-NFQ-3′	56	AbD
16	SNP	Exon 7	Pro339Leu	C/T		2,811	5′-AGCCGCTCAGATCTTCTTCTCT-3′	5′-TTGAACTTGTTGTAGCTAGCAAAAGC-3′	5′-VIC-CAAAGCCCGGACCAA-MGB-NFQ-3′	5′-FAM-CAAAGCCCAGACCAA-MGB-NFQ-3′	75	AbD
17	SNP	Exon 8	Leu362Met	C/A		1,504	5′-CGGCCCCTTGGGTTTTC-3′	5′-GAAGCTCGTCATGCAGTTCAC-3′	5′-VIC-CCAGAGATGCCCTGGTGA-MGB-NFQ-3′	5′-FAM-CAGAGATGCCATGGTGA-MGB-NFQ-3′	68	AbD
18	SNP	Exon 9	Ile425Val	A/G		8,181	5′-GCAGAAGCGATAGCCAACATG-3′	5′-CAAGCCCAGCGTGATTAACATC-3′	5′-VIC-TTTCTTTGCCATCATCTT-MGB-NFQ-3′	5′-VIC-TCTTTGCCGTCATCTT-MGB-NFQ-3′	78	AbD
19	SNP	Exon 13	Lys605Asn	A/C		4,687	5′-TCCCCACATATATAGCTTATCGGTTGA-3′	5′-CAAAACAATTAGTAGTCTGAACACACACA-3′	5′-VIC-CACGTACCTCTTTAAAT-MGB-NFQ-3′	5′-FAM-ACGTACCTCGTTAAAT-MGB-NFQ-3′	105	AbD
20	SNP	Exon 14	Pro621Ser	C/T		…	5′-GCGTATTATTAAAAGTATTACCCCAGAAACAC-3′	5′-CACAGCATTCAAGCGGATGTC-3′	5′-VIC-CACAAGGAATTTCT-MGB-NFQ-3′	5′-FAM-CACAAGAAATTTCT-MGB-NFQ-3′	73	AbD

^aFrom the Single Nucleotide Polymorphism Web site.